Publication details

Authors: Shekhar, Sudhanshu; Lee, Boeun; Roy, Abhijit; Candiello, Joe; Kumta, Prashant N. 
Title: Surface mediated non-viral gene transfection on titanium substrates using polymer electrolyte and nanostructured silicate substituted calcium phosphate pDNA (NanoSiCaPs) composites 
Type: Journal Article 
Publisher: Materials Today Communications  
Year: 2018 
Volume: 16 
Start Page: 169-173 
End Page:  
DOI: 10.1016/j.mtcomm.2018.05.008 
Abstract: Exploiting gene delivery from the surfaces of bio-functionalized implants is a unique strategy to facilitate tissue regeneration and integration. However, it has been challenging, due to the difficulty for incorporation and delivey of sufficient payloads of plasmid DNA (pDNA) to cells from the implant surface allowing efficient gene expression. Herein, we describe a novel and simple coating approach using nanostructured silicate substituted calcium phosphate pDNA complexes termed as NanoSiCaPs, that preferentially adsorb onto titanium (Ti) substrates coated with poly (diallyldimethylammonium chloride) (PDADMAC). The Ti-polyelectrolyte NanoSiCaPs assemblies aptly called PNA, deliver pDNA to MC3T3-E1 cells (pre-osteoblast cell line), induce protein production, without eliciting any cytotoxicity, while simultaneously encouraging cell attachment on the substrate.  
Keywords: Calcium phosphate (CaP) 
File:  (1257K)